pneumoniae from other Streptococcus species. The bile solubility test based on spectrophotometric reading described in this study can differentiate S. pseudopneumoniae strains but misidentified 13 other mitis group strains. Visual spectra evaluation correctly identified all S. pseudopneumoniae but misidentified 16 other mitis group strains. MALDI-TOF score value identification identified correctly 46 S. mitis isolate generated an OD-value above 2.1). pneumoniae from all but one other mitis group streptococci (one S. Applying a calculated optimal cut-off absorbance-value of 2.1, differentiated S.
Eighty-four species verified mitis group isolates were subjected to our bile solubility test (which measures and calculates the differences of absorbance in the test tube containing 10% sodium deoxycholate versus a blank control tube, after incubation for 10 minutes at 36 ☌ using a spectrophotometer) and MALDI-TOF MS (both the standard result output and by visual spectra evaluation). pneumoniae from Streptococcus pseudopneumoniae. This study assesses a novel bile solubility test and MALDI-TOF for the differentiation of Streptococcus pneumoniae from other mitis group streptococci, including differentiation of S.
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